Economic Importance Viruses in this genus are collectively of worldwide importance causing significant economic losses on a wide range of crops. The Tomato spotted wilt virus genome is processed differentially in its plant host Arachis hypogaea and its thrips vector Frankliniella fusca.
Nothing significant to report during this reporting period. Information on the molecular variability of DMV would facilitate the further development of detection tools that could identify a wide range of DMV variants.
Using a set of indicator plants, biologically distinct strains of thrips-transmitted Iris yellow spot virus IYSVa serious viral pathogen of onion and other Alliums were characterized.
Additional viruses have also been identified from vegetables and ornamentals in the United States, Israel and Brazil eg. Prior toTSWV was considered a monotypic group of plant viruses. We are successful in isolating and mass-producing selected DMV proteins.
The diversity in the genomic sequences of DMV is being used to develop broad spectrum detection tools such as antisera and PCR primers. New viruses in this group continue to be identified. These receptors are likely implicated in transmission through their ability to confer specificity to the virus—vector interaction.
Evaluation of lisianthus Eustoma grandiflorum as an indicator host for Iris yellow spot virus. A rapid, PCR-based assay was developed for broad spectrum detection of the members of genus Caulimovirus.
Published online May Incidence and relative distribution of distinct caulimoviruses Genus Caulimovirus, Family Caulimoviridae associated with dahlia mosaic in Dahlia variabilis. Results showed that PVS infection resulted in breakdown of late blight resistance.
Tospoviruses are "emerging " viruses not only in the sense of their increasing economic importance as pathogens on a worldwide basis, but also in that our understanding of the complexity of this new viral taxon only began to emerge during the last decade.
Determine the role of various caulimoviruses in pathogenicity in dahlia and characterize the 35S homologs in these caulimoviruses Project Methods Tomato spotted wilt virus and Iris yellow spot virus will be used as model systems to study the interaction of these viruses with selected hosts.
First report of Iris yellow spot virus infecting garlic and leek in Zimbabwe. In Advances in Virus Research, ed. Symptom development and distribution of Tomato spotted wilt virus in flue-cured tobacco. New geographical record of Tomato spotted wilt virus; improved understanding of protein-protein interactions of virally coded proteins and their potential role in viral life cycle; evaluation of onion cultivars for resistance to Iris yellow spot virus IYSV ; molecular epidemiology of IYSV; evaluation of systemic acquired resistance against IYSV; small RNA profiles of TSWV wild type and mutants and an increased understanding of the host response to TSWV infection.
APSnet Image of the Week. Evaluation of onion relatives for virus resistance is a pre-requisite for selecting breeding material for producing virus-resistant cultivars or hybrids.
Although tospoviruses can be transmitted mechanically in the lab with varying efficiency, in the field they are transmitted from plant to plant by specific thrips species order Thysanoptera, Family Thripidae in a persistent, propagative manner. Serological and molecular assays for rapid and sensitive detection of Iris yellow spot virus Genus Tospovirus, Family Bunyaviridae infection of bulb and seed onion crops.
Iris yellow spot virus epidemics continued to cause economic losses in bulb and seed onion crops in the western U. Incidence of this virus in onion in Hawaii was confirmed.
Impacts Improved diagnostics for Dahlia mosaic virus in dahlia would help detect and intercept virus-infected production stock from being distributed; rapid and sensitive detection of Iris yellow spot virus in onion enabled us to determine its presence in other production areas of the state, these techniques are being used to evaluate IYSV management programs that are in field trials.
Development of serotype-specific antisera is in progress. As part of an ongoing project, the prevalence of various Barley yellow dwarf virus BYDV serotypes extant in wheat and other grassy hosts in Washington was determined. The emerging problem of tospvirus infection and nonconventional methods of control.
As a member of the multi-state, collaborative effort on diagnosis, epidemiology and control of IYSV in bulb and seed onion crops, a Feature Article was prepared and published in Plant Disease. Magnetic resonance imaging for the detection of viral infections in potato tubers.
Phylogenetic analyses show that the EPRSs from various sources form one clade indicating a lack of clustering by geographical origin.
New weed hosts of IYSV were identified. Although host ranges tend to vary from virus to virus, Nicotiana benthamiana is a good assay host for most Tospoviruses. To identify sources of resistance to IYSV, several onion relatives were screened for virus susceptibility.
Concurrently, Seepiban, Gajanandana, Attathom, and Attathom identified the same virus as the causal agent of necrotic spots, necrotic ringspots, and stem necrosis in tomato and pepper plants in Nakhon Pathom province, Thailand, and showed that it could be transmitted by both Thrips palmi and C.
Continue to train and mentor MS and PhD students and post-doctoral fellows Impacts What was accomplished under these goals?Tospoviruses have a very wide host range and can be transmitted by ten different thrips species.
Using disease-free and disease-resistant tomato transplants is the best way to prevent the spread of tospoviruses. Tospovirus.
Tospoviruses are exclusively transmitted by thrips and cause devastating diseases of many economically important crops worldwide (Maris et al., ). The WFT is currently considered the most efficient vector of these viruses (German et al.,Ullman et al., ).
Thesis: Tospovirus – Induction and Suppression of RNA silencing Supervirors: Richard Kormelink, Rob Goldbach and Just Vlak Postdoctoral Researcher at Embrapa Vegetables.
Embrapa. Wageningen University & Research. Visualizar perfil. Visualizar crachás de perfis. Pesquisar por mi-centre.com: Postdoctoral Researcher at. TSWV, the type species of the genus Tospovirus, occurs worldwide, because of its wide plant host range and the worldwide distribution of its main vector, the thrips Frankliniella occidentalis.
Other members of the Tospovirusgenus appear more restricted in their distribution (Table 1). Generally, tospoviruses are widespread in tropical, subtropical. Progress 01/01/09 to 12/31/09 Outputs OUTPUTS: Research focus continued on thrips-transmitted tospoviruses in vegetables, and plant pararetroviruses in ornamentals.
Biological and molecular characterization of Iris yellow spot virus (IYSV), the most economically important tospovirus in the region was carried out. In the latter instance, they were in mixed infection with either the potyvirus ArLV or the tospovirus Tomato spotted wilt virus (TSWV) in plants showing severe stunting, leaf malformation, and necrosis.Download